Polymerase Specific Error Rates and Profiles Identified by Single Molecule Sequencing

Publication date: Available online 19 January 2016 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Matthew S. Hestand, Jeroen Van Houdt, Francesca Cristofoli, Joris R. Vermeesch DNA polymerases have an innate error rate which is polymerase and DNA context specific. Historically the mutational rate and profiles have been measured using a variety of methods, each with their own technical limitations. Here we used the unique properties of single molecule sequencing to evaluate the mutational rate and profiles of six DNA polymerases at the sequence level. In addition to accurately determining mutations in double strands, single molecule sequencing also captures direction specific transversions and transitions through the analysis of heteroduplexes. Not only did the error rates vary, but also the direction specific transitions differed among polymerases.

Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - January 19, 2016 Category: Cytology Source Type: research