Bioaffinity Mass Spectrometry Screening

Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS or ESI-FTMS) was used to screen 192 natural product extracts and a 659-member natural product-based fragment library for bindings to a potential malaria drug target, Plasmodium falciparum Rab11a (PfRab11a, PF13_0119). One natural product extract and 11 fragments showed binding activity. A new natural product, arborside E, was identified from the active extract of Psydrax montigena as a weak binder. Its binding activity and inhibitory activity against PfRab11a were confirmed by ESI-FTMS titration experiments and an orthogonal enzyme assay.

Source: Journal of Biomolecular Screening - January 15, 2016 Category: Molecular Biology Authors: Yang, B., Feng, Y. J., Vu, H., McCormick, B., Rowley, J., Pedro, L., Crowther, G. J., Van Voorhis, W. C., Forster, P. I., Quinn, R. J. Tags: Original Research Source Type: research