Hypoxia-induced alterations of G2 checkpoint regulators

Hypoxia promotes an aggressive tumor phenotype with increased genomic instability, partially due to downregulation of DNA repair pathways. However, genome stability is also surveilled by cell cycle checkpoints. An important issue is therefore whether hypoxia also can influence the DNA damage-induced cell cycle checkpoints. Here, we show that hypoxia (24h 0.2% O2) alters the expression of several G2 checkpoint regulators, as examined by microarray gene expression analysis and immunoblotting of U2OS cells.

Source: Molecular Oncology - January 8, 2016 Category: Cancer & Oncology Authors: Grete Hasvold, Christin Lund-Andersen, Malin Lando, Sebastian Patzke, Sissel Hauge, ZhenHe Suo, Heidi Lyng, Randi G. Syljuåsen Source Type: research