Biotin-conjugated fusogenic liposomes for high-quality cell purification

In this study, we developed a new approach for efficient cell population separation based on the specific membrane fusion characteristics of distinct cell types upon treatment with fusogenic liposomes. When such liposomes are conjugated with biotin, specific cell populations can be efficiently surface functionalized by biotin after liposomal treatment while other populations remain unlabeled. Due to the high affinity of biotin for avidin-like proteins, biotin functionalized cells are ideal targets for conjugation of e.g. avidin tagged magnetic beads, fluorophores or antibodies with bioanalytical relevance. Here, based on the differential biotinylation of distinct cell populations high quality separation of cardiac fibroblasts from myocytes, and cerebromicrovascular endothelial cells from fibroblasts was successfully established.

Source: Journal of Biomaterials Applications - January 5, 2016 Category: Materials Science Authors: Hersch, N., Wolters, B., Ungvari, Z., Gautam, T., Deshpande, D., Merkel, R., Csiszar, A., Hoffmann, B., Csiszar, A. Tags: Original Articles Source Type: research