Enzymatic thiol Michael addition using laccases: Multiple CS bond formation between p-hydroquinones and aromatic thiols

Publication date: Available online 7 January 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Max Schlippert, Annett Mikolasch, Veronika Hahn, Frieder Schauer Laccases create CC, CO or CN bonds and have been investigated intensively as catalysts for green chemistry and white biotechnology. However, little is known about CS bond formation in laccase-catalyzed reactions. We have used the laccases from Pycnoporus cinnabarinus and Myceliophthora thermophila to create heteromolecular reaction products from p-hydroquinones and aromatic thiols via CS bond formation. We discovered a broad assortment of different thiolated products, ranging from dimers to tetramers. During laccase-catalysis of the aromatic thiols without p-hydroquinones homomolecular dimers were formed and these were always linked by SS bonds. The use of laccase is a convenient way to form heteromolecular thiolated products and homomolecular SS bonds under eco-friendly environments. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research