GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.

GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.
Curr Protoc Hum Genet. 2016;88:15.8.1-15.8.12
Authors: Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M
Abstract
Transgenic technologies used for creating a desired genomic change in animals involve three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them and their subsequent transfer to recipient females. These ex vivo steps have been widely used for over 3 decades and they were also readily adapted for the latest genome editing technologies such as ZFNs, TALENs, and CRISPR/Cas9 systems. We recently developed a method called GONAD (Genome editing via Oviductal Nucleic Acids Delivery) that does not require all the three critical steps of transgenesis and therefore relieves the bottlenecks of widely used animal transgenic technologies. Here we provide protocols for the GONAD system. © 2016 by John Wiley & Sons, Inc.
PMID: 26724720 [PubMed - in process]

Source: Current Protocols in Human Genetics - January 11, 2016 Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research