Preparation of rodent testis co-cultures.
Preparation of rodent testis co-cultures.
Curr Protoc Toxicol. 2013 Feb;Chapter 16:Unit 16.10
Authors: Wegner S, Hong S, Yu X, Faustman EM
Abstract
Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a protocol for preparation of a three-dimensional in vitro model of male reproductive development that reduces the number of animals required for evaluation of toxicants. A Matrigel overlay provides a three-dimensional extracellular matrix that improves cell attachment, viability, and communication, and makes the model more reflective of in vivo environments.
PMID: 23408194 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Toxicology - Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research
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