Using the cytosensor microphysiometer to assess ocular toxicity.

Using the cytosensor microphysiometer to assess ocular toxicity.
Curr Protoc Toxicol. 2014;61:1.13.1-1.13.11
Authors: Nash JR, Mun G, Raabe HA, Curren R
Abstract
Measuring in vitro cytotoxicity is one method currently used to estimate damage to the eye after chemical exposure. The Cytosensor Microphysiometer method evaluates cytotoxicity by measuring the test material-induced reduction in the metabolic rate of L929 cells. Changes in metabolic rate are measured indirectly as a function of changes in the extracellular acidification rate of the cells. During exposure to increasing concentrations of a cytotoxic material, there is a decrease in the release of acid byproducts into the surrounding medium as the cells die. These acidic metabolic byproducts cause a measurable change in the pH of a lightly buffered medium, which can be measured by the Cytosensor Microphysiometer. The change in the pH of the medium over time is then converted into a metabolic rate estimate for the cells. The endpoint measurement from the assay is the metabolic rate decline of 50%, the MRD50 value (in units of mg/ml). Curr. Protoc. Toxicol. 61:1.13.1-1.13.11. © 2014 by John Wiley & Sons, Inc.
PMID: 25104266 [PubMed - in process]

Source: Current Protocols in Toxicology - December 21, 2015 Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research