High-Resolution Multi-Photon Imaging of Morphological Structures of Caenorhabditis elegans.

High-Resolution Multi-Photon Imaging of Morphological Structures of Caenorhabditis elegans.
Curr Protoc Toxicol. 2015;64:11.19.1-11.19.11
Authors: Bixel GM, Fretham SJ, Aschner M
Abstract
In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Caenorhabditis elegans (C. elegans). Femto-second laser pulses excite second-harmonic generation (SHG) and third-harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two-photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans. © 2015 by John Wiley & Sons, Inc.
PMID: 26344221 [PubMed - in process]

Source: Current Protocols in Toxicology - December 21, 2015 Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research