Pitfalls in PCR troubleshooting: Expect the unexpected?

Publication date: Available online 10 December 2015 Source:Biomolecular Detection and Quantification Author(s): Livia Schrick, Andreas Nitsche PCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here we report a PCR troubleshooting that came up with a surprising result never observed before. With this report we hope to sensitize the reader to this peculiar problem and to save troubleshooting efforts in similar situations, especially in time-critical and ambitious diagnostic settings.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research