Aspergillus fumigatus Cap59-like protein A is involved in {alpha}1,3-mannosylation of GPI-anchors

In this study, we characterized an α1,3-mannosyltransferase involved in maturation of GPI-anchors from the pathogenic fungus Aspergillus fumigatus. This enzyme shows homology to Cryptococcus neoformans Cap59p, a putative glycosyltransferase involved in capsule formation and virulence, and was thus named Cap59-like protein A (ClpA). Targeted deletion of the clpA gene in A. fumigatus led to absence of α1,3-mannose from mature GPI-anchors. The enzyme was further located to the Golgi-like apparatus of A. fumigatus and was shown to be active in the yeast Saccharomyces cerevisiae.

Source: Glycobiology - December 7, 2015 Category: Biology Authors: Krüger, A. T., Engel, J., Buettner, F. F. R., Routier, F. H. Tags: Glycan Synthesis Source Type: research