Cheap and environmentally sustainable stereoselective arylketones reduction by Lactobacillus reuteri whole cells

Publication date: Available online 3 December 2015 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): F.M. Perna, M.A. Ricci, A. Scilimati, M.C. Mena, I. Pisano, L. Palmieri, G. Agrimi, P. Vitale Various Lactobacillus reuteri strains were investigated as whole cell catalysts for the bioreduction of acetophenone into optically active (R)-1-phenylethanol. Lactobacillus reuteri DSM 20016 strain gave an almost complete substrate conversion, in a short reaction time and enantiomeric excess up to 99%. The acetophenone bioreduction was used as a model reaction to optimize temperature and reducing equivalent source (glucose, lactose, cheese way and lignocellulosic hydrolysates) to accomplish the biotransformation. The reduction of acetophenones into optically active (R)-1-arylethanols was also exploited to study L. reuteri DSM 20016 substrate specificity. In most of the cases, optically active (R)-1-arylethanols have been obtained with both excellent chemical and optical yields and with (R)-enantiopreference, through a cheap, simple and efficient process. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research