Enhancement of enzymatic synthesis of sucrose 6-acetate with Aspergillus oryzae fructosyltransferase using ionic liquid as a cosolvent

Publication date: Available online 1 December 2015 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Tao Wei, Weiwei Jia, Xuan Yu, Duobin Mao The aim of this study was to investigate the effects of using ionic liquids (ILs) as cosolvents on the enzymatic synthesis of sucrose 6-acetate (S6A) by Aspergillus oryzae fructosyltransferase (AoFT). Fifteen ILs were selected as IL-containing buffer systems to assist the catalytic reactions in the AoFT-catalyzed synthesis of S6A. An analysis of the catalytic efficiency and conformation of AoFT showed that the [Dmim][PF6]–phosphate buffer (20:80, v/v) system was the optimum medium for the AoFT-catalyzed production of S6A. The maximum glucose 6-acetate (G6A) conversion and S6A yield were obtained under the following conditions: reaction time 8h, reaction temperature 55°C, AoFT concentration 20mg/mL, molar ratio of sucrose to G6A 4:1, and sucrose concentration 10mg/mL. The reaction time decreased 0.33-fold, and the G6A conversion and S6A yield increased 2.90-fold and 3.18-fold, respectively, under these conditions. These results show that the use of ILs as cosolvents enhances the transfructosylation activity of AoFT in the enzymatic synthesis of S6A. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research