Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation

Nicotiana benthamiana has shown great success as a platform for the production of recombinant proteins. Here, we describe methods to transiently express high levels of recombinant proteins and simultaneously modulate their glycosylation pattern toward human-like structures. The method aims to generate recombinant proteins with a targeted largely homogeneous glycosylation profile for structure–function studies.

Source: Springer protocols feed by Plant Sciences - December 1, 2015 Category: Biology Source Type: news