P133

Publication date: November 2015 Source:European Journal of Cancer Supplements, Volume 13, Issue 1 Author(s): A. Cherepanova, V. Vlassov, P. Laktionov The strong connection between cancer-related inflammation and tumour development with pattern – recognizing receptors (PRRs) activation results in identification of new target molecules that could lead to improved cancer diagnosis and treatment. TLR3, RIG1 and MDA5 synthetic ligand poly(I:C) was shown to trigger apoptosis in cancer cells. However, TLR3 signaling also includes NF-kB transcription factor which has emerged as endogenous tumour promoter via stimulation of pro-inflammatory tumour microenvironment, enhancement of angiogenesis, tumour cell proliferation and metastasis. Thus down regulation of NF-kB-mediated effects after TLR3 activation is needed for implementation of TLR3 ligand-based therapy into clinical trials. We have earlier demonstrated that DNA and sequence specific ODNs inhibit poly(I:C)-induced production of pro-inflammatory cytokines in human primary fibroblasts and endothelial cells (Cherepanova et al., Immunobiology, 2013). Using these specific ODNs and affinity modification/isolation approach combined with subsequent MALDI-TOF the main cellular targets for these ODNs were identified as Ku protein – heterodimer of KU70 and KU80 (Cherepanova et al., Exp. Opin. Biol. Ther., 2012). The goals of this study are to reveal whether the ODNs target poly(I:C)-induced activation in tumour cells: cervica...
Source: European Journal of Cancer Supplements - Category: Cancer & Oncology Source Type: research