T116

Publication date: November 2015 Source:European Journal of Cancer Supplements, Volume 13, Issue 1 Author(s): B. Biedermann The work describes advantages of our latest instrument developments in flow cytometry and microscopy. Characteristics of newly launched flow cytometer, which uses acoustic focusing to align the cells in the focus of the laser for analysis, allows to analyze samples at a much higher throughput rate without losing precision and sensitivity. This opens up new possibilities regarding sample preparation and analysis. These advantages we want to demonstrate on 2 examples, whole blood analysis and rare event analysis. Analysis of biological samples in the most physiologic state with minimal sample preparation and manipulation is a key objective to any workflow. However as whole blood samples generally require significant manipulation, such as wash/centrifugation steps and/or red blood cell lysis, we have developed multiple no-lyse, no-wash assays to minimize these manipulation steps to obtain more physiological results. Here we show how we utilize the rapid sample collection capabilities of the our instrument to characterize phagocyte function in human whole blood with a phagocytosis/phagosome acidification assay and also a dihydrorhodamine 123 superoxide production assay, in a no-lyse, no wash format. Another area where our technology can shift boundaries is the field of rare event analysis. Due to the low frequency of the target cell population a large n...
Source: European Journal of Cancer Supplements - Category: Cancer & Oncology Source Type: research