Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice.

Implanted neonatal human dermal fibroblasts influence the recruitment of endothelial cells in mice. Biomatter. 2012 Jan-Mar;2(1):43-52 Authors: Guerreiro SG, Brochhausen C, Negrão R, Barbosa MA, Unger RE, Kirkpatrick CJ, Soares R, Granja PL Abstract The vascularization of new tissue within a reasonable time is a crucial prerequisite for the success of different cell- and material-based strategies. Considering that angiogenesis is a multi-step process involving humoral and cellular regulatory components, only in vivo assays provide the adequate information about vessel formation and the recruitment of endothelial cells. The present study aimed to investigate if neonatal human dermal fibroblasts could influence in vivo neovascularization. Results obtained showed that fibroblasts were able to recruit endothelial cells to vascularize the implanted matrix, which was further colonized by murine functional blood vessels after one week. The vessels exhibited higher levels of hemoglobin, compared with the control matrix, implanted without fibroblasts, in which no vessel formation could be observed. No significant differences were detected in systemic inflammation. The presence of vessels originated from the host vasculature suggested that host vascular response was involved, which constitutes a fundamental aspect in the process of neovascularization. Fibroblasts implanted within matrigel increased the presence of endothelial cells with posit...
Source: Biomatter - Category: Biotechnology Tags: Biomatter Source Type: research