Mechanical and biocompatible characterization of a cross-linked collagen-hyaluronic acid wound dressing.

Mechanical and biocompatible characterization of a cross-linked collagen-hyaluronic acid wound dressing. Biomatter. 2013 Oct-Dec;3(4) Authors: Kirk JF, Ritter G, Finger I, Sankar D, Reddy JD, Talton JD, Nataraj C, Narisawa S, Millán JL, Cobb RR Abstract Collagen scaffolds have been widely employed as a dermal equivalent to induce fibroblast infiltrations and dermal regeneration in the treatment of chronic wounds and diabetic foot ulcers. Cross-linking methods have been developed to address the disadvantages of the rapid degradation associated with collagen-based scaffolds. To eliminate the potential drawbacks associated with glutaraldehyde cross-linking, methods using a water soluble carbodiimide have been developed. In the present study, the glycosaminoglycan (GAG) hyaluronic acid (HA), was covalently attached to an equine tendon derived collagen scaffold using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) to create ntSPONGE The HA was shown to be homogeneously distributed throughout the collagen matrix. In vitro analyses of the scaffold indicated that the cross-linking enhanced the biological stability by decreasing the enzymatic degradation and increasing the thermal denaturation temperature. The material was shown to support the attachment and proliferation of mouse L929 fibroblast cells. In addition, the cross-linking decreased the resorption rate of the collagen as measured in an intramuscular implant model in rabbits....
Source: Biomatter - Category: Biotechnology Tags: Biomatter Source Type: research