Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues.

Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues. Curr Protoc Cytom. 2012 Jul;Chapter 6:Unit 6.33 Authors: Xie J, Ferbas J, Juan G Abstract Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed. PMID: 22752952 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Cytometry - Category: Molecular Biology Tags: Curr Protoc Cytom Source Type: research