How to build a time-gated luminescence microscope.

How to build a time-gated luminescence microscope. Curr Protoc Cytom. 2014;67:Unit 2.22. Authors: Jin D, Lu Y, Leif RC, Yang S, Rajendran M, Miller LW Abstract The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence. PMID: 24510771 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Cytometry - Category: Molecular Biology Tags: Curr Protoc Cytom Source Type: research