The application of KillerRed for acute protein inactivation in living cells.

The application of KillerRed for acute protein inactivation in living cells. Curr Protoc Cytom. 2014;69:12.35.1-12.35.10 Authors: Jarvela TS, Linstedt AD Abstract Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live-cell imaging of cultured cells. PMID: 24984963 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Cytometry - Category: Molecular Biology Tags: Curr Protoc Cytom Source Type: research