Influence of prednisolone on glucose and uric acid transport across peritoneal membrane in vitro.

Influence of prednisolone on glucose and uric acid transport across peritoneal membrane in vitro. Adv Perit Dial. 2012;28:21-5 Authors: Grzelak T, Czyzewska-Majchrzak L, Kramkowska M, Wojciechowska K, Szary B, Witmanowski H, Czyzewska K Abstract Prednisolone and other glucocorticosteroids are used by some peritoneal dialysis patients because of underlying diseases such as peritonitis. Although corticosteroids are potent inhibitors of various processes during inflammation, their influence on the transport properties of peritoneum is little known. Our study investigated the influence of prednisolone (0.001 g/dL) on glucose (1.8 g/dL) and uric acid (0.02 g/dL) transfer across isolated parietal peritoneum taken from the anterior abdominal wall of white Hyplus 59 rabbits and placed inside a modified Ussing-type chamber. Values for transfer from the interstitial (I) to the mesothelial (M) side of membrane (I-->M) and in the opposite direction (M-->I) were calculated using the mathematical model of mass transport and are expressed as a coefficient of diffusive permeability [P (in centimeters per second)]. Four separate series of experiments were done. In the first and second series, we respectively examined glucose transport under control conditions (for 120 minutes) and then before (15-60 minutes) and after (75-120 minutes) introduction of prednisolone on the M side of the membrane. In the third and fourth series, similar studies of ...
Source: Advances in Peritoneal Dialysis. Conference on Peritoneal Dialysis - Category: Urology & Nephrology Tags: Adv Perit Dial Source Type: research