Preparation of HCV NS3 and NS5B proteins to support small-molecule drug discovery.

Preparation of HCV NS3 and NS5B proteins to support small-molecule drug discovery. Curr Protoc Pharmacol. 2011 Sep;Chapter 13:Unit13B.6 Authors: Hung M, Wang R, Liu X Abstract Production of high-quality, well-characterized recombinant proteins facilitates screening of compound libraries. The protocols detailed in this unit are used to purify three recombinant enzymes that are widely used in HCV research: the HCV NS3 protease domain, the helicase domain as an NS3+NS4A complex, and the NS5B RNA-dependent RNA polymerase. The active enzymes are purified to homogeneity by two-column chromatography to support a screening program for HCV inhibitors. PMID: 21898331 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Pharmacology - Category: Drugs & Pharmacology Tags: Curr Protoc Pharmacol Source Type: research