Follicle-stimulating hormone–induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes

The effect of follicle-stimulating hormone (FSH) on apoptotic status of cumulus cells, expression of proapoptotic and antiapoptotic genes, and development rate of in vitro fertilization–produced buffalo embryos were investigated. FSH supplementation in in vitro maturation–medium resulted in a dose-dependent reduction in the expression of proapoptotic genes namely, BCL2-associated X protein (BAX), cytochrome c, and caspase-3 and increase in the expression of antiapoptotic genes such as B-cell lymphoma 2 (BCL2) and X-linked inhibitor of apoptosis protein (XIAP) in cumulus cells of mature oocyte.
Source: Domestic Animal Endocrinology - Category: Endocrinology Authors: Source Type: research