Porcine cholecyst derived extracellular matrix (p-CEM) for reconstruction of full thickness skin wounds in rats

Publication date: Available online 12 November 2015 Source:Wound Medicine Author(s): Poonam Shakya, A.K. Sharma, Naveen Kumar, V. Remya, Dayamon D. Mathew, Prasoon Dubey, Kiranjeet Singh, Sonal, Sameer Shrivastava, S.K. Maiti, Anwarul Hasan, K.P. Singh An acellular cholecyst derived extracellular matrix (p-CEM) of porcine origin was prepared using anionic biological detergent. Healing potential of (p-CEM) was compared with commercially available collagen sheet (b-CS) and open wound (C) in full-thickness skin wounds in rats. Thirty six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full-thickness skin wound (20X20 mm2) was produced on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (p-CEM). Healing was evaluated on the basis of wound contraction, gross, haematological, immunological and histopathological observations. On critical analysis of the observed parameters it was found that p-CEM has healing potential and shown better healing response than commercially available collagen sheet to reconstruct full thickness skin wounds in rats.
Source: Wound Medicine - Category: Journals (General) Source Type: research