Isolation and Functional Analysis of Human Neutrophils.

Isolation and Functional Analysis of Human Neutrophils. Curr Protoc Immunol. 2015;111:7.23.1-7.23.16 Authors: Kuhns DB, Priel DA, Chu J, Zarember KA Abstract This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described. © 2015 by John Wiley & Sons, Inc. PMID: 26528633 [PubMed - in process]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research