Molecular Characterization of Myf5 and Comparative Expression Patterns of Myogenic Regulatory Factors in Siniperca Chuatsi

Publication date: Available online 4 November 2015 Source:Gene Expression Patterns Author(s): Xin Zhu, Yu-Long Li, Li Liu, Jian-Hua Wang, Hong-Hui Li, Ping Wu, Wu-Ying Chu, Jian-She Zhang Myogenic regulatory factors (MRFs) are muscle-specific basic helix–loop–helix (bHLH) transcription factor that plays an essential role in regulating skeletal muscle development and growth. To investigate molecular characterization of Myf5 and compare the expressional patterns of the four MRFs, we cloned the Myf5 cDNA sequence and analyzed the MRFs expressional patterns using quantitative real-time polymerase chain reaction in Chinese perch (Siniperca chuatsi). Sequence analysis indicated that Chinese perch Myf5 and other MRFs shared a highly conserved bHLH domain with those of other vertebrates. Sequence alignment and phylogenetic tree showed that Chinese perch MRFs had the highest identity with the MRFs of Epinephelus coioides. Spatio-temporal expressional patterns revealed that the MRFs were primarily expressed in muscle, especially in white muscle. During embryonic development period, Myf5, MyoD and MyoG mRNAs had a steep increase at neurula stage, and their highest expressional level was predominantly observed at hatching period. Whereas the highest expressional level of the MRF4 was observed at the muscular effect stage. The expressional patterns of post-embryonic development showed that the Myf5, MyoD and MyoG mRNAs were highest at 90 days post-hatching (dph). F...

Source: Gene Expression Patterns - November 4, 2015 Category: Genetics & Stem Cells Source Type: research