Interleukin-6 increases the in vitro expression of key proteins associated with steroidogenesis in the bovine adrenal zona fasciculata

In this study, the in vitro effects of interleukin-6 (IL-6) on the mRNAs and proteins for key steroidogenic factors in the bovine adrenal zona fasciculate (ZF) were determined. Bovine adrenal glands were obtained from an abattoir and the ZF isolated. Strips of ZF were then exposed to different concentration of murine IL-6 and/or adrenocorticotropic hormone (ACTH) for various time intervals, the protein and mRNA extracted, and the mRNA and protein expression determined by RT-PCR and western blots.

Source: Domestic Animal Endocrinology - October 26, 2015 Category: Endocrinology Authors: S. McIlmoil, J. Strickland, A.M. Judd Source Type: research