Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus

Rapid amplification of cDNA ends (RACE) is a technique that was developed to swiftly and efficiently amplify full-length RNA molecules in which the terminal ends have not been characterized. Current usage of this procedure has been more focused on sequencing and characterizing RNA 5′ and 3′ untranslated regions. Herein is described an adapted RACE protocol to amplify bacterial RNA transcripts.

Source: Springer protocols feed by Microbiology - October 22, 2015 Category: Microbiology Source Type: news