Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products

Genetic manipulation of bacteria often requires the joining together of more than one DNA segment to form a hybrid DNA molecule. This can be accomplished by PCR followed by restriction endonuclease digestions and ligations. However, this approach can often become laborious and expensive. Here is described a well-established method for using primer design and PCR to obtain hybrid products for use in cloning vectors, mutagenesis protocols, and other applications.

Source: Springer protocols feed by Microbiology - October 22, 2015 Category: Microbiology Source Type: news