Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species

The ability to extract, identify and annotate large amounts of biological data is a key feature of the “omics” era, and has led to an explosion in the amount of data available. One pivotal advance is the use of Next-Generation Sequencing (NGS) techniques such as RNA-sequencing (RNA-seq). RNA-seq uses data from millions of small mRNA transcripts or “reads” which are aligned to a reference genome. Comparative transcriptomics analyses using RNA-seq can provide the researcher with a comprehensive view of the cells’ response to a given environment or stimulus.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news