Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.
Source: Springer protocols feed by Immunology - Category: Allergy & Immunology Source Type: news