A General Method for Measuring Persister Levels in Escherichia coli Cultures

Genetically homogeneous bacterial cultures contain persisters, cells that are not killed by bactericidal antibiotics. These cells are suggested to be involved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.
Source: Springer protocols feed by Immunology - Category: Allergy & Immunology Source Type: news