NMR Approaches to Functional Dynamics of Genetically Separated iGluR Domains

We describe here the production and isotopic labeling of the ligand-binding domain of an AMPA receptor (GluA2) to permit studies of protein dynamics by NMR. The strategies for resonance assignment and the experiments used to study dynamics are also described.
Source: Springer protocols feed by Neuroscience - Category: Neuroscience Source Type: news