Studying the Translatome with Polysome Profiling

Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.

Source: Springer protocols feed by Genetics/Genomics - October 16, 2015 Category: Genetics & Stem Cells Source Type: news