Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells

We describe the application of a CRISPR-Cas9 effector system from Streptococcus pyogenes for transcriptional regulation in mammalian cells resulting in activation or repression of transcription. We present methods for appropriate target site selection, sgRNA design, and delivery of dCas9 and dCas9-effector system components into cells through lentiviral transgenesis to modulate transcription.

Source: Springer protocols feed by Genetics/Genomics - October 16, 2015 Category: Genetics & Stem Cells Source Type: news