Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.

We describe in depth the latest version of our monolayer chemically defined small molecule differentiation protocol, including metabolic selection-based cardiomyocyte purification and the addition of triiodothyronine to enhance cardiomyocyte maturation. Finally, we describe a method for the dissociation of hPS cell-derived cardiomyocytes, cryopreservation, and thawing. © 2015 by John Wiley & Sons, Inc. PMID: 26439715 [PubMed - in process]

http://www.ncbi.nlm.nih.gov/pubmed/26439715?dopt=Abstract

Source: Current Protocols in Human Genetics - October 8, 2015 Category: Genetics & Stem Cells Tags: Curr Protoc Hum Genet Source Type: research