Schisandrae Fructus Inhibits IL‐1β‐Induced Matrix Metalloproteinases and Inflammatory Mediators Production in SW1353 Human Chondrocytes by Suppressing NF‐κB and MAPK Activation

This study investigated the antiosteoarthritis properties of an ethanol extract of SF on IL‐1β‐stimulated SW1353 chondrocytes. SF attenuated IL‐1β‐induced expression and activity of matrix metalloproteinase (MMP)‐1, MMP‐3, and MMP‐13 and also reduced the elevated levels of cyclooxygenase‐2 and inducible nitric oxide synthase associated with the inhibition of prostaglandin E2 and nitric oxide production in IL‐1β‐stimulated SW1353 chondrocytes. In addition, SF markedly suppressed the nuclear translocation of nuclear factor‐kappa B (NF‐κB) by blocking inhibitor κB‐alpha degradation and inhibited the phosphorylation of c‐Jun N‐terminal kinase (JNK) and p38 mitogen‐activated protein kinase (MAPK). These results indicate that the inhibitory effect of SF on IL‐1β‐stimulated expression of MMPs and inflammatory mediators production in SW1353 cells were associated with the suppression of the NF‐κB and JNK/p38 MAPK signaling pathways. The results from this study indicate that SF may have therapeutic potential for the treatment of OA due to its anti‐inflammatory and chondroprotective features. Drug Dev Res, 2015. © 2015 Wiley Periodicals, Inc.
Source: Drug Development Research - Category: Drugs & Pharmacology Authors: Tags: Research Article Source Type: research