Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.

Source: Springer protocols feed by Genetics/Genomics - October 2, 2015 Category: Genetics & Stem Cells Source Type: news