Role of the Na+/H+ exchanger 3 in angiotensin II-induced hypertension

The renal mechanisms responsible for angiotensin II (ANG II)-induced hypertension remain incompletely understood. The present study tested the hypothesis that the Na+/H+ exchanger 3 (NHE3) is required for ANG II-induced hypertension in mice. Five groups of wild-type (Nhe3+/+) and Nhe3–/– mice were treated with vehicle or high pressor doses of ANG II (1.5 mg/kg/day ip, via minipump for 2 wk, or 10 pmol/min iv for 30 min). Under basal conditions, Nhe3–/– mice had significantly lower systolic blood pressure (SBP) and mean intra-arterial pressure (MAP) (P < 0.01), 24 h urine (P < 0.05), urinary Na+ (P < 0.01) and urinary K+ excretion (P < 0.01). In response to ANG II, SBP and MAP markedly increased in Nhe3+/+ mice in a time-dependent manner, as expected (P < 0.01). However, these acute and chronic pressor responses to ANG II were significantly attenuated in Nhe3–/– mice (P < 0.01). Losartan blocked ANG II-induced hypertension in Nhe3+/+ mice but induced marked mortality in Nhe3–/– mice. The attenuated pressor responses to ANG II in Nhe3–/– mice were associated with marked compensatory humoral and renal responses to genetic loss of intestinal and renal NHE3. These include elevated basal plasma ANG II and aldosterone and kidney ANG II levels, salt wasting from the intestines, increased renal AQP1, Na+/HCO3–, and Na+/K+-ATPase expression, and increased PKCα, mitogen-activated protein kinases ER...

Source: Physiological Genomics - October 1, 2015 Category: Genetics & Stem Cells Authors: Li, X. C., Shull, G. E., Miguel-Qin, E., Zhuo, J. L. Tags: Molecular Genetics of Complex Traits Source Type: research