Enhancing Cognate Target Elution Efficiency in Gel-Free Chemical Proteomics

Publication date: Available online 30 September 2015 Source:EuPA Open Proteomics Author(s): Branka Radic-Sarikas, Uwe Rix, Alexey Stukalov, Manuela Gridling, André C. Müller, Jacques Colinge, Giulio Superti-Furga, Keiryn L. Bennett Gel-free liquid chromatography mass spectrometry coupled to chemical proteomics is a powerful approach for characterizing cellular target profiles of small molecules. We have previously described a fast and efficient elution protocol; however, altered target profiles were observed. We hypothesised that elution conditions critically impact the effectiveness of disrupting drug-protein interactions. Thus, a number of elution conditions were systematically assessed with the aim of improving the recovery of all classes of proteins whilst maintaining compatibility with immunoblotting procedures. A double elution with formic acid combined with urea emerged as the most efficient and generically applicable elution method for chemical proteomics Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research