Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5′-Rapid Amplification of cDNA Ends (5′-RACE)

Transcription start sites are commonly used to locate promoter elements in bacterial genomes. TSS were previously studied one gene at a time, often through 5′-rapid amplification of cDNA ends (5′-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in microorganisms.

Source: Springer protocols feed by Genetics/Genomics - September 15, 2015 Category: Genetics & Stem Cells Source Type: news