Comparison of early and delayed transplantation of adipose tissue-derived mesenchymal stem cells on pulmonary arterial function in monocrotaline-induced pulmonary arterial hypertensive rats

The aim of this article was to investigate the effects of early and delayed transplantation of adipose tissue-derived mesenchymal stem cells (ADMSCs) on pulmonary vasomotion in monocrotaline (MCT)-induced pulmonary arterial hypertensive (PAH) rats. Adipose tissue-derived mesenchymal stem cells were isolated from inguinal subcutaneous adipose tissue of Sprague-Dawley (SD) rats. Eighty male SD rats were divided into four groups (n = 20 per group): normal control (Ctr), PAH, early (at day 7, ADMSCs-7), and delayed (at day 14, ADMSCs-14) ADMSC intervention. PAH was induced by a single dose intraperitoneal injection of 40 mg/kg MCT. About 1 x 106 ADMSCs were delivered via left external jugular vein. Mean pulmonary arterial pressure (MPAP) was measured by catheterization at days 21 and 28 after MCT injection. Pulmonary arteriolar endothelium-dependent relaxation (EDdR), endothelium-independent relaxation (EDiR), and vasoconstriction function were evaluated by the physiological vascular ring tension recording system. Mean pulmonary arterial pressure (mmHg) was increased on days 21 (26.88 ± 0.86 vs. 14.88 ± 0.98, P < 0.05) and 28 (32.22 ± 0.71 vs. 15.16 ± 0.95, P < 0.05) after MCT administration; EDdR and EDiR were lower in PAH rats at the 21st day (pD2:EDdR: 4.85 ± 0.29 vs. 8.12 ± 0.48 and EDiR: 6.28 ± 0.38 vs. 8.64 ± 0.35; all P < 0.05) and 28th day (pD2:EDdR: 3.22 ± 0.61 vs. 7.93 ± 0.5 and pD2:EDiR: 5.4...
Source: European Journal of Heart Failure Supplements - Category: Cardiology Authors: Tags: Articles Source Type: research