Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers

Visualization of actin cytoskeletal dynamics is critical for understanding the spatial and temporal regulation of actin remodeling. Drosophila oogenesis provides an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in Drosophila egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.

Source: Springer protocols feed by Cell Biology - September 5, 2015 Category: Cytology Source Type: news