A new practical guide to the Luria-Delbrück protocol

Publication date: Available online 28 August 2015 Source:Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Author(s): Qi Zheng Since 2000 several review papers have been published about the analysis of experimental data obtained using the Luria-Delbrück protocol. These timely papers cleared much of the confusion surrounding various methods for estimating or comparing mutation rates. As a result, today the fluctuation test is more widely applied with much improved accuracy. The present paper provides guidelines on a few remaining problems that continue to baffle mutation researchers. Among the issues addressed are incomplete plating, relative fitness, and comparison of experiments where average final cell population sizes differ. It also offers a fresh view on the estimation methods that are based on the sample median.

Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - August 30, 2015 Category: Cytology Source Type: research