Control for stochastic sampling variation and qualitative sequencing error in next generation sequencing

Conclusion In targeted NGS, synthetic competitive IS control for stochastic sampling at input of both target into library preparation and of target library product into sequencer, and control for qualitative errors generated during library preparation and sequencing. These controls enable accurate clinical diagnostic reporting of confidence limits and limit of detection for copy number measurement, and of frequency for each actionable mutation.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research