Human FAN1 promotes strand incision in 5'-flapped DNA complexed with RPA

In this study, we purified human FAN1, as a bacterially expressed recombinant protein. FAN1 exhibited robust endonuclease activity with 5'-flapped DNA, which is formed at the stalled replication fork. We found that FAN1 efficiently promoted DNA incision at the proper site of RPA-coated 5'-flapped DNA. Therefore, FAN1 possesses the ability to promote the ICL repair of 5'-flapped DNA covered by RPA.

Source: Journal of Biochemistry - August 29, 2015 Category: Biochemistry Authors: Takahashi, D., Sato, K., Hirayama, E., Takata, M., Kurumizaka, H. Tags: Regular Papers Source Type: research