Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice

Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.

Source: International Immunology - August 26, 2015 Category: Allergy & Immunology Authors: Yamashita, N., Jitsuki-Takahashi, A., Ogawara, M., Ohkubo, W., Araki, T., Hotta, C., Tamura, T., Hashimoto, S.-i., Yabuki, T., Tsuji, T., Sasakura, Y., Okumura, H., Takaiwa, A., Koyama, C., Murakami, K., Goshima, Y. Tags: Short communication Source Type: research