In vitro analysis of dextran from Leuconostoc mesenteroides NRRL B-1426 for functional food application

Publication date: Available online 24 August 2015 Source:Bioactive Carbohydrates and Dietary Fibre Author(s): Damini Kothari, Jagan Mohan Rao Tingirikari, Arun Goyal The present study describes physico-chemical properties, prebiotic potential and biocompatibility of dextran produced from Leuconostoc mesenteroides NRRL B-1426. Dextran displayed porous surface, pseudoplastic non-Newtonian behaviour, 32% solubility, 290% water holding capacity and a degradation temperature of 290°C. The dextran exhibited significantly higher non digestibility as compared with the standard prebiotic, inulin in simulated human gastric juice and α-amylase. The dextran significantly stimulated the growth of probiotics, bifidobacteria and lactobacilli and was comparable to that by inulin. The viability of human embryonic kidney (HEK 293), human cervical adenocarcinoma (INT-407) and human colon carcinoma (HT-29) cell lines by dextran treatment (50–1000µg/ml) remained unaffected displaying the biocompatibility of the dextran. Therefore, the dextran from Leuconostoc mesenteroides NRRL B-1426 can act as potential prebiotic, gelling agent and be used as food supplement for health benefits. Graphical abstract
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research