Expression of Recombinant pET22b-LysK-CHAP Bacteriophage therapeutic Protein in

Bacteriophage-encoded endolysins are a group of enzymes that act by digesting the peptidoglycan of bacterial cell walls. LysK has been reported to lyses live staphylococcal cultures. LysK proteins containing only the CHAP domain has capability to show lytic activity against live clinical staphylococcal isolates, including MRSA. The aim of study was cloning and expression of LysK-CHAP domain in E.coli Bl21 DE3 by using pET22-b as a secretion vector. The pET22b plasmid was used that encode a pelB secretion signal under control of the strong bacteriophage T7 promoter.
Source: Osong Public Health and Research Perspectives - Category: Global & Universal Authors: Tags: Original Article Source Type: research